Part:BBa_K2220026:Design

Express mCherry-alpha-tubulin fusion protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2119
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1093
  Illegal BsaI site found at 2101
  Illegal BsaI.rc site found at 1799
  Illegal SapI site found at 957
  

Design Notes

We have done the codon optimization.

Source

Alpha-factor comes from pyes2/ct/alpha-factor plasmid. Alpha-tubulin: http://www.ncbi.nlm.nih.gov/nuccore/393715088